PDFs of Publications


Malacrida et al.,  The phasor plot: a universal circle to advance fluorescence lifetime analysis and interpretation.  Ann Rev Biophysics  2021

Hedde, et al., (2021) Membrane Remodeling by Arc/Arg3.1.  Frontiers in Molecular Biosciences 8, 20

Gunther et al. LAURDAN since Weber: the quest for visualizing membrane heterogeneity. ACR 2021


Vallmitjana et al. _Resolution of 4 components in the same pixel in FLIM images using the phasor approach._Methods_Appl._Fluoresc._8_035001 2020

Castro-Castillo et al CAPRYDAA, an anthracene dye analog to LAURDAN: a comparative study using cuvette and microscopy. J Mater Chem B 2020

Albanesi et al. Palmitoylated Proteins in Dendritic Spine Remodeling. Front Synaptic Neurosci 2020


James et al.  Characterization of clostridium botulinum neurotoxin serotype A (BoNT/A) and fibroblast growth factor receptor interactions using a novel receptor dimerization assay.  BioRxiv 2019


Barylko et al. Palmitoylation and Membrane Binding of Arc/Arg3.1: A Potential Role in Synaptic Depression.  Biochemistry 2018

Gunther et al. Scanning fluorescence correlation spectroscopy comes full circle. Methods 2018

Ranjit et al. Fit-free analysis of fluorescence lifetime imaging data using the phasor approach. Nat Protocols 2018

Gallea et al. Amyloid oligomerization of the Parkinson’s disease related protein α-synuclein impacts on its curvature-membrane sensitivity. J Neurochem 2018

Kumagai et al. Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions. Extremophiles 2018


Nguyen et al  Higher Order Oligomerization of the Licensing ORC4 Protein Is Required for Polar Body Extrusion in Murine Meiosis.  J Cell Biochem. 2017

Malacrida et al A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes. Sci Reports 2017


Lopes et al Environmental Factors Modulating the Stability and Enzymatic Activity of the Petrotoga mobilis Esterase (PmEst). PLoS One. 2016 Jun 28;11(6):e0158146 (2016)

Jameson  A Fluorescent Lifetime: Reminiscing About Gregorio Weber in Perspectives in Fluorescence: A Tribute to Gregorio Weber.   Prspectives on Fluorescence 2016


Malacrida et al        Model-free methods to study membrane environmental probes: A comparison of the spectral phasor and generalized polarization approaches. Methods Appl Fluores 2015

Lopes et al        Investigation of the conformational flexibility of DGAT1 peptides using tryptophan fluorescence. Methods Appl Fluores 2015

Byers et al        Enhancement of dynamin polymerization and GTPase activity by Arc/Arg3.1. BBA 2015 


Ranjit et al        Application of three-photon FCS to the study of protein oligomerization.  J Phys Chem B 2014

Reilley et al        Folding and hydrodynamics of a DNA i-motif from the c-MYC promoter determined by fluorescent cytidine analogs. Biophys J 2014

Montecinos-Franjola et al        Single tryptophan mutants of FtsZ: Nucleotide binding/exchange and conformational transitions. BBA 2014

Vetromile and Jameson        Frequency domain fluorometry: theory and application. Methods Mol Biol 2014

James and Jameson        Steady-state fluorescence polarization/anisotropy for the study of protein interactions. Methods Mol Biol 2014

James et al        A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells. BBA 2014


Jameson et al        Investigations of protein-protein interactions using time-resolved fluorescence and phasors. Methods 2013

Jameson et al        Fluorescence Fluctuation Analysis Approaches to the Study of Receptors in Live Cells. Methods Enzymology 2013


James et al        Number and Brightness Analysis of LRRK Oligomerization in Live Cells. Biophys J 2012

Montecinos-Franjola et al        Spontaneous Dissociation and Unfolding of E. Coli FtsZ Supports the Dimer Nucleus Polymerization Model. Biophys J 2012

Buscaglia et al        G-Quadruplex structure and stability illuminated by 2-aminopurine phasor plots. Nucleic Acids Res 2012


Zakharov et al        Guanidinium chloride-induced spectral perturbations of 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid confound interpretation of data on molten globule states. Analytical Biochem 2011

Gilmore et al        Depolarization after resonance energy transfer (DARET): A sensitive fluorescence-based assay for botulinum neurotoxin protease activity. Analytical Biochem 2011

Ross et al         Characterization of Förster resonance energy transfer in a botulinum neurotoxin protease assay. Analytical Biochem 2011

Ross et al        Dimeric Endophilin A2 Stimulates Assembly and GTPase Activity of Dynamin 2. Biophys J 2011

Ross et al        Oligomerization State of Dynamin 2 in Cell Membranes Using TIRF and Number and Brightness Analysis. Biophys J 2011

James et al        Application of Phasor Plots to In Vitro Protein Studies. Analytical Biochem 2011

Stefl et al        Application of Phasor Plots to In Vitro Time-Resolved Fluorescence Measurements. Analytical Biochem 2011


Barylko et al        The Proline-Arginine Rich Domain (PRD) is a Major Determinant of Dynamin Self-Activation. Biochemistry 2010

Wang et al        Dynamin 2 mutants linked to centronuclear myopathies form abnormally stable polymers. J Biol Chem 2010

Jameson and Ross        Fluorescence Polarization/Anisotropy in Clinical Diagnostics and Imaging. Chem Rev 2010

James et al        Excited-State Lifetime Studies of the Three Tryptophan Residues in the N-Lobe of Human Serum Transferrin. Protein Sci 2010


Jameson et al        Fluorescence Fluctuation Spectroscopy: Ushering in a New Age of Enlightenment in Cellular Dynamics. Biophys Rev 2009

Martin et al        Fluorescence Characterization of Chemical Microenvironments in Hydrophobically Modified Chitosan. Carbo Poly 2009


Ross and Jameson        Frequency Domain Fluorometry: Applications to Intrinsic Protein Fluorescence. J Photochem Photobiol Sci 2008

García-Marcos et al        Yeast Ribosomal Stalk Composition and Dynamics Studied In Vivo by Two-Photon Fluorescence Correlation Spectroscopy and Molecular Brightness Analysis. Biophys J 2008


Elsey et al        Fluorescent measurement of microalgal neutral lipids. J. Micro. Methods 2007


Cuevas et al         Modulation of reconstituted pig kidney Na+/K+-ATPase activity by cholesterol in endogenous lipids vesicles : Role of lipid domains. Biochemistry 2006

Konash et al        Characterization of polymer-enzyme interactions. J Mater Chem 2006

Arbidua et al        Fluorescence resonance energy transfer and molecular modeling studies on 4′,6-diamidino-2-phenylindole (DAPI) complexes with tubulin. Protein Sci 2006


Barbieri et al        Frequency-domain fluorescence spectroscopy using 280-nm and 300-nm light-emitting diodes: Measurement of proteins and protein-related fluorophores. Analytical Biochem 2005

Jameson and Mocz        Fluorescence polarization/anisotropy approaches to study protein-ligand interactions: effects of errors and uncertainties. Methods Mol Biol 2005

Eccleston et al        Fluorescence-based assays. Prog Med Chem 2005

Moens et al        Oligomeric state and mode of self-association of Thermotoga maritima ribosomal stalk protein L12 in solution. Biochemistry 2005


Harwell et al        Effects of Surface Passivation on Silicon Nanoparticle Photoluminescence. Chem Lett 2004

Moens et al        Detection of Tryptophan to Tryptophan Energy Transfer in Proteins. Protein J 2004

Sanchez et al        Tubulin equilibrium unfolding followed by time-resolved fluorescence and fluorescence correlation spectroscopy. Protein Sci 2004


Shevyakov et al        Orbital Control of the Color and Excited State Properties of Formylated and Fluorinated Derivatives of Azulene. J Phys Chem A  2003  

Croney et al        Fluorescence Resonance Energy Transfer Studies on Anthrax Lethal Toxin. FEBS Lett 2003

Croney et al        Conformational Dynamics and Temperature Dependence of Photo-induced Electron Transfer within Self-Assembled Coproporphyrin:Cytochrome c Complexes. Biophys J 2003

Jameson and Croney        Fluorescence Polarization: Past, Present and Future. Comb High Throughput Chem 2003

Oiwa et al        The 2’-O- and 3’-O-Cy3-EDA-ATP(ADP) Vanadate Complexes with Myosin S1 are Spectroscopically Distinct. Biophys J 2003

Jameson et al        Fluorescence: Basic Concepts, Practical Aspects and Some Anecdotes. Methods Enzymol 2003


Tramier et al         Binding of Ethidium to Yeast tRNAPhe : A New Perspective on an Old Bromide 2002 in Fluorescence Spectroscopy, Imaging and Probes (Eds. R. Kraayenhof, A.J.W.G. Visser & H.C. Gerritsen). Springer, Berlin, pp.111-120.

Bagatolli et al        Activation of Dynamin II by POPC in Giant Unilamellar Vesicles: A Two-Photon Fluorescence Microscopy Study. J Prot Chem 2002

Blackman et al         Structural and Biochemical Characterization of a Fluorogenic Rhodamine-Labeled Malarial Protease Substrate. Biochemistry 2002

Voss et al        Discrete Bathochromic Shifts Exhibited by Fluorescein Ligand Bound to Rabbit Polyclonal Anti-Fluorescein Fab Fragments. J Prot Chem 2002

Eccleston et al        Oligomerization and Kinetic Mechanism of the Dynamin GTPase. Eur Biophys J 2002


Jasuja et al         Temperature Dependence of Photoinudced Electron Transfer within Self-Associated Porphyrin:Guanine Monophosphate Complexes. Chem Phys Lett 2001

Jameson        The Seminal Contributions of Gregorio Weber to Modern Fluorescence Spectroscopy2001 in New Trends in Fluorescence Spectroscopy, eds B. Valeur and J.-C. Brochon, Springer-Verlag, Berlin, pp.35-58.

Croney et al        Teaching Basic Fluorescence Principles with Simple Visual Demonstrations. J Biochem Edu 2001


Sotomayor et al        Modulation of pig kidney (Na,K)-ATPase activity by cholesterol: Role of hydration. Biochemistry 2000

Binns et al        The Mechanism of GTP Hydrolysis by Dynamin II – a Transient Kinetic Study. Biochemistry 2000

Croney et al        Temperature Dependence of Photo-Induced Electron Transfer within Self-Assembled Uroporphyrin:Cytochrome c Complexes. J Phys Chem 2000


Jameson and Seifried         Quantification of Protein-Protein Interactions Using Fluorescence Polarization. Methods 1999

Castanho et al          The Pentaene Antobiotic Filipin Prefers More Rigid DPPC Bilayers: A Fluorescence Pressure Dependence Study. BBA 1999

Binns et al        Correlation Between Self-Association Modes and GTPase Activation of Dynamin. J Prot Chem 1999

Larsen et al        Ground and Excited State Characterization of an Electrostatic Complex between Tetrakis-(4-Sulphanatophenyl) Porphyrin and 16-Pyrimidium Crown-4. Photochem Photobiol 1999

Larsen et al         Spectroscopic Characterization of Two Soluble Transducers from the Archeon Halobacterium salinarum. J Prot Chem 1999

Lasagna et al        Apohorseradish Peroxidase Unfolding and Refolding: Intrinsic Tryptophan Fluorescence Studies. Biophys J 1999


Sanchez et al        Aggregation States of Mitochondrial Malate Dehydrogenase. Protein Sci 1998

Jameson         Gregorio Weber, 1916-1997: A Fluorescent Lifetime. Biophys J 1998

Helms et al          Site -Directed Mutants of Rat Testis Fructose 6-Phosphate, 2-Kinase:Fructose 2,6-Bisphosphatase: Localization of Conformational Alterations Induced by Ligand Binding. Biochemistry 1998

Mocz et al          Probing the Nucleotide Binding Site of Axonemal Dynein with the Fluorescent Nucleotide Analog 2′(3′)-O-(N-methylanthrananiloyl)-adenosine 5′-triphosphate. Biochemistry 1998

Barylko et al         Synergistic Activation of Dynamin GTPase by Grb2 and Phosphoinositides. J Biol Chem 1998


Larsen et al         Conformational Modulation of Electron Transfer within Electrostatic Porphyrin:Cytochrome c Complexes.  J Phys Chem B 1997

Helms et al         Time-Resolved Fluorescence Studies on Site-Directed Mutants of Human Serum Albumin. FEBS Lett 1997

Jasuja et al         Singlet Excited State Dynamics Associated with Nucleosides/Nucleotides Complexed with Tetrakis(4-N-methyl)pyridyl)porphine. J Phys Chem B 1997

Jameson and Eccleston         Fluorescent Nucleotide Analogs: Synthesis and Applications. Methods Enzymol 1997


Gratton et al        Fluorescence Dynamics of Biological Systems Using Synchrotron Radiation. Rev Sci Instrum 1996

Hamman et al        Tetramethylrhodamine Dimer Formation as a Spectroscopic Probe of the Conformation of Escherichia Coli Ribosomal Protein L7/L12 Dimers. J Biol Chem 1996

Hamman et al         Rotational and Conformational Dynamics of Escherichia Coli Ribosomal Protein L7/L12 Biochemistry 1996

Hamman et al         Dimer/Monomer Equilibrium and Subunit Exchange of Escherichia Coli Ribosomal Protein L7/L12. Biochemistry 1996

Helms et al         Dynamics and Morphology of the In Vitro Polymeric Form of Elongation Factor Tu from Escherichia coli. BBA 1996

Petersen et al         Structural Requirements of a Specific Human Albumin Thyroxine Binding Site. J Biol Chem 1996

Watanabe et al         Fluorescence Studies of Four Single Tryptophan Mutants of Rat Testies Fructose 6-Phosphate, 2-Kinase:Fructose 2,6-Bisphosphatase. Protein Sci 1996

Pouchnik et al         Synthesis and Spectral Characterization of Sulfhydryl-Reactive Fluorescent Probes. Analytical Biochem 1996

Lasagna et al         Spectral Properties of Environmentally Sensitive Probes Associated with Horseradish Peroxidase. Biochemistry 1996

Larsen et al         Spectroscopic and Molecular Modeling Studies of Caffeine Complexes with DNA-Intercalators. Biophys J 1996


Traut et al         Location and Domain Structure of Escherichia Coli Ribosomal Protein L7/L12: Site Specific Cysteine Crosslinking and Attachment of, Fluorescent Probes. Biochem Cell Biol 1995

Watson et al           The Arrangement of Macromolecules in the Aminoacyl-tRNA•Elongation Factor Tu•GTP Ternary Complex as Determined by Fluorescence Energy Transfer. Biochemistry 1995

Lim et al         Molecular Dynamics of Anti-Fluorescein 4-4-20 Antigen-Binding Fragment: Time-Resolved Fluorescence Spectroscopy. Biochemistry 1995

Jameson and Sawyer         Fluorescence Anisotropy Applied to Biomolecular Interactions. Methods Enzymol 1995

Helms et al         Polymerization of Escherichia coli Elongation Factor Tu. BBA 1995


Tominaga et al       Reversible Unfolding of Fructose 6-Phosphate, 2-Kinase:Fructose 2,6-Bisphosphatase. Protein Sci 1994

Uauy et al         Safety and Efficacy of Omega-3 Fatty Acids in the Nutrition of Very Low Birth Weight Infants: Soy Oil and Marine Oil Supplementation of Formula.  J Pediatrics 1994

Brunet et al         Hydrodynamics of Horseradish Peroxidase Revealed by Global Analysis using Multiple Fluorescent Probes. Biophysical J 1994


Hazlett et al         Solution Dynamics of P21 Ras Proteins Bound with Fluorescent Nucleotides: A Time-Resolved Fluorescence Study. Biochemistry 1993

Dughi et al         Fluorescence Investigations of Albumin from Patients with Familial Dysalbuminemic Hyperthyroxinemia. Photochem Photobiol 1993


Treen et al         Effect of Docosahexanoic Acid on Membrane Fluidity and Function in Intact Cultured Y-79 Retinoblastoma Cells. Arch Biochem Biophys 1992

Williams et al         Amino Acid Profiles and Liposomes: Their Role as Chemosensory Information Carriers in the Marine Environment. J Chem Ecology 1992


Reinhart et al         A Method for On-Line Background Subtraction in Frequency Domain Fluorometry.   J Fluorescence 1991

Jameson and Hazlett         Time-Resolved Fluorescence Measurements in Biology and Biochemistry. in, Biophysical and Biochemical Aspects of Fluorescence Spectroscopy. ed. G. Dewey, Plenum Press, N.Y., pp 105-133. 1991

Vargas et al         Oxygen Diffusion Near the Heme Binding Site of Horseradish Peroxidase. BBRC 1991


Yoo et al           Fluorescence Studies of Nucleotide Interactions with Bovine Adrenal Chromogranin A. BBA 1990

Vandermeulen et al         Excitation Wavelength Dependent Fluorescence Anisotropy of Eosin-Myosin Adducts: Evidence for Anisotropic Rotations. Biophys Chem 1990

Brunet et al         Oxygen Diffusion Through Horseradish Peroxidase. Photochem Photobiol 1990


Jullian et al         Time-Resolved Fluorescence Studies on Protoporphyrin IX – Apohorseradish Peroxidase. BBA 1989

Voelker et al         In Vitro Evidence that Urine Composition Affects the Fraction of Active Furosemide in the Nephrotic Syndrome. J Pharm Exp Therap 1989

Hazlett et al         Time-Resolved Fluorescence Studies on the Ternary Complex Formed Between Bacterial Elongation Factor Tu, Guanosine 5′-Triphosphate, and Phenyl¬alanyl-tRNAPPhe Biochemistry 1989

Jameson et al         Time-Resolved Fluorescence Studies on NADH Bound to Mitochondrial Malate Dehydrogenase. BBA 1989

Levi et al        Role of Brush Border Membrane Lipid Composition and Fluidity in Impaired Renal Phosphate Transport in the Aged Rat Kidney. Am J Physiol 1989

Laws and Jameson         Time-Resolved Fluorescence in Studies of Protein Structure and Dynamics. In, Synchrotron Radiation in Structural Biology: Brookhaven Symp. Biol. 35, eds. R.M. Sweet and A.D. Woodhead, Plenum Press., N. Y., pp. 207-219. 1989